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Biological functions of ferroptosis-related genes in ccRCC cells. (A) CCK-8 kit assay showed that OSRC2 and <t>CAKI2</t> cells viability increased after silencing EPAS1 and ALDH3A2, while decreased after silencing BID, AURKA and PLA2G6. (B) Wound healing assay experiments showed that knocking down EPAS1 and ALDH3A2 led to increase of invasive OSRC2 and CAKI2 cells, while decreased after silencing BID, AURKA and PLA2G6. (C) Plate colony formation assay showed that colony formation of OSRC2 and CAKI2 cells increased after silencing EPAS1 and ALDH3A2, while decreased after silencing BID, AURKA and PLA2G6. (Scalebar-100 μm *p < 0.05, **p < 0.01, and ***p < 0.001, n.s no significance, according to Student’s t-test).
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Biological functions of ferroptosis-related genes in ccRCC cells. (A) CCK-8 kit assay showed that OSRC2 and CAKI2 cells viability increased after silencing EPAS1 and ALDH3A2, while decreased after silencing BID, AURKA and PLA2G6. (B) Wound healing assay experiments showed that knocking down EPAS1 and ALDH3A2 led to increase of invasive OSRC2 and CAKI2 cells, while decreased after silencing BID, AURKA and PLA2G6. (C) Plate colony formation assay showed that colony formation of OSRC2 and CAKI2 cells increased after silencing EPAS1 and ALDH3A2, while decreased after silencing BID, AURKA and PLA2G6. (Scalebar-100 μm *p < 0.05, **p < 0.01, and ***p < 0.001, n.s no significance, according to Student’s t-test).

Journal: Frontiers in Pharmacology

Article Title: The biological function and prognostic significance of ferroptosis-related genes in clear cell renal cell carcinoma

doi: 10.3389/fphar.2025.1515552

Figure Lengend Snippet: Biological functions of ferroptosis-related genes in ccRCC cells. (A) CCK-8 kit assay showed that OSRC2 and CAKI2 cells viability increased after silencing EPAS1 and ALDH3A2, while decreased after silencing BID, AURKA and PLA2G6. (B) Wound healing assay experiments showed that knocking down EPAS1 and ALDH3A2 led to increase of invasive OSRC2 and CAKI2 cells, while decreased after silencing BID, AURKA and PLA2G6. (C) Plate colony formation assay showed that colony formation of OSRC2 and CAKI2 cells increased after silencing EPAS1 and ALDH3A2, while decreased after silencing BID, AURKA and PLA2G6. (Scalebar-100 μm *p < 0.05, **p < 0.01, and ***p < 0.001, n.s no significance, according to Student’s t-test).

Article Snippet: The OSRC2 and CAKI2 cell lines, commonly employed in renal cancer research ( ; ; ; ), were obtained from the American Type Culture Collection (ATCC).

Techniques: CCK-8 Assay, Wound Healing Assay, Colony Assay